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Image Search Results
Journal: APL Bioengineering
Article Title: An inexpensive programmable optogenetic platform for controlled neuronal activation regimens in C. elegans
doi: 10.1063/1.5120002
Figure Lengend Snippet: Overview of stimulation and imaging platform. (a) Single-animal stimulation and tracking. Worms are illuminated from below using a brightfield source and imaged at 4× magnification, optionally illuminated with the LED lamp. Imaging procedures include intermittent lamp use, and neuronal stimulation regimens require only the PC, Arduino, DC power supply, and LED lamp. For imaging procedures, a MATLAB program simultaneously sends signals to the Arduino microcontroller and records images via the CCD camera. When the Arduino receives the signal to power the LED lamp, it activates the relay, which routes power from the power supply through the LED driver to the lamp. The LED driver provides the LED lamp with a constant current. The system is placed on a stereoscope, with base brightfield illumination, and an objective and CCD camera at the top. The diagram represents the light path and information flow; microscope components appear rotated 90° counterclockwise in this schematic. (b) Whole population stimulation. The setup is the same as in (a), except no imaging is performed, and the plate is covered with aluminum foil.
Article Snippet: MATLAB 2018b was used, and our MATLAB script used the Image Processing Toolbox, Image Acquisition Toolbox, MATLAB Support Package for
Techniques: Imaging, Microscopy